![]() ![]() Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Sizing of proteins on SDS-polyacrylamide gels and western blots.Monitoring protein transfer onto membranes after western blotting.Description The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. Thermo Scientific PageRuler Unstained Protein Ladder is a mixture of 14 recombinant, highly purified, unstained proteins (10 to 200 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. Monitoring protein migration during SDS-polyacrylamide gel electrophoresis. Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / 9 bands / SDS-polyacrylamide gel electrophoresis Categories: Protein Marker/Ladder.Use the ladder for easy visualization of gel migration and protein size, and to orient your gel or membrane quickly. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. Includes 11 protein bands from 8 to 260 kDa (see figure). Thermo Scientific Spectra Multicolor Broad Range Protein Ladder is a 4-color (blue, orange, green, pink) protein standard containing 10 prestained proteins (10 to 260 kDa) for use in gel electrophoresis and western blotting. Membrane-compatible-colored bands transfer to membranes for Western blotting. The Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection.Two reference bands-orange at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.Quality tested-each lot evaluated by SDS-PAGE and Western blotting.Sharp bands-color-coded bands of similar intensity for easy visualization.Ready-to-use-supplied in a loading buffer for direct loading on gels no need to boil.Size range-10 proteins spanning from 10 to 180 kDa. ![]() The ladder contains one orange reference band at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa. This prestained protein marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Not intended for human, animal diagnostic or therapeutic uses.ABP Prestained Protein Marker is a mixture of ten blue, orange or green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein marker is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use. Pre-Stained Protein Marker (9-245 kDa) is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Western blotting protein standards can be used for both fluorescent visualization and colormetric or chemiluminescent immunodetection on western blots. 5l of IRIS11 Prestained Protein Ladder (PMI11) resolves 11 bands in 4-20 SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. Additional data should be considered for a more accurate adjustment.Īpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. These protein standards and ladders are consistent from lot to lot and are strictly. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. 2.5 μl per well for general Western transferring. Refer to the Blu13 (BLUelf) Prestained Protein Ladder patterns in various electrophoresis conditions:ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Guide for Molecular Weight Estimation (kDa) The Blu13 Prestained Protein Ladder (BLUelf) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu13 Prestained Protein Ladder (BLUelf) is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |